About the Instrument
| Scope: | Inverted Axiovert 200 M SP |
| Objectives: | DIC C-Apochromat 63X/1.2W; DIC Plan-Neo 25X/0.8 IMM; Plan-Apo 10X/0.45 WD=2.1; |
| VIS Laser: | Argon Laser 458/477/488/514nm, 30mW; HeNe Laser 543nm, 1mW; HeNe Laser 633nm, 5mW; temperature-stabilized VIS-AOTF for simultaneous; |
| UV Laser: | Argon UV laser 351/364nm, 80mW; temperature-stabilized UV-AOTF for simultaneous intensity control of two ultraviolet laser lines |
| Scanning resolution: | 4 x1 to 2048 x 2048 pixels, continuous adjustment; |
| Scanning speed: | 13 x 2 speed stages; up to 5 frames/s with 512 x 512 pixels (max. 77 frames/s with 512 x 32 pixels); min. 0.38 ms for a line of 512 pixels |
| Scanning zoom: | 0.7x to 40 x, digital, variable in steps of 0.1 |
| Scanning rotation: | Free 360° rotation, variable in steps of 1 degree, free xy offset |
| Detection: | Three confocal epi-illumination channels simultaneously (META detector + 2 single-channel detectors), each with a high-sensitivity PMT detector. One transmitted-light channel with PMT; |
| META detector: | Polychromatic 32-channel detector for fast acquisition of Lambda Stacks and Metatracking |
| Data depth: | Selectable between 8 bit and 12 bit |
| Computer: | Intel Pentium IV processor with 1.7GHz; 3.7GB memory, workstation, TFT flat-panel 1280X1024 pixels duel monitors; |
| Analysis: | Advanced tools for colocalization and histogram analysis with individual parameters and options, profile measurement of straight lines and curves of any shape, measurement of lengths, angles, areas, intensities, etc. |
| Image operations: | Addition, subtraction, multiplication, division, ratio, shift, filters (low-pass, median, high-pass, etc.; user-definable) |
| Data archiving: | LSM image database with convenient functions for managing images together with their acquisition parameters |
| Export and Import: | Multiprint function for creating assembled image and data views; more than 20 file formats (TIF, BMP, JPG, PSD, PCX, GIF, AVI, Quicktime …) for compatibility with all common image processing programs |